Figure 5. RNA editing levels within the spliced nad1 chimeric transcript.

The SDx construct was electroporated into isolated mitochondria as described in Materials and Methods. After 18 hr incubation, the mtRNA was isolated and the chimeric spliced products were obtained by RT-PCR using primers located in the exon nad1b and the terminator cob. The PCR product inserted into the pGEM-T vector was cloned and sequenced. Editing levels of C-targets on exons nad1b to nad1e represent the average of 29 independent clones (gray bars). The positions of C targets in the mature nad1 transcript, considering the first nucleotide the beginning ATG codon from exon nad1a, are indicated below the bars.