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. 2012 Dec 20;7(12):e52644. doi: 10.1371/journal.pone.0052644

Table 1. Editing status of nad1e and nad5c intermediates.

Transcript edited molecules (N° clones)
nad1e precursor 50% (10)
nad1e branched intermediate 100% (24)
nad5c precursor 40% (10)
nad5c branched intermediate 100% (36)

The PCR products from branched intermediates obtained with primers P2a and P3a specific for nad1e and primers P2b and P3b specific for nad5c were purified from agarose gel and cloned into pGEM-T, subsequently, selected clones were sequenced on both strands.