Table 1. Editing status of nad1e and nad5c intermediates.
Transcript | edited molecules (N° clones) |
nad1e precursor | 50% (10) |
nad1e branched intermediate | 100% (24) |
nad5c precursor | 40% (10) |
nad5c branched intermediate | 100% (36) |
The PCR products from branched intermediates obtained with primers P2a and P3a specific for nad1e and primers P2b and P3b specific for nad5c were purified from agarose gel and cloned into pGEM-T, subsequently, selected clones were sequenced on both strands.