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Histologically, a mixture of keratinous material and stratified squamous epithelium is required to diagnose cholesteatoma. The epithelium displays stratified squamous epithelium with keratinization (matrix). The subepithelial region is occupied by connective tissue. Abbreviations: C: stratum corneum; G: stratum granulosum; S: stratum spinosum; B: stratum basale; D: dermis. Magnification, 600x.
