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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Brain Res Bull. 2012 Sep 26;90:35–42. doi: 10.1016/j.brainresbull.2012.09.009

Figure 2. Ascorbate effects on NE generation from relatively high DA concentrations.

Figure 2

Cells in culture were incubated in the absence (circles) or presence (squares) of 500 μM DHA, followed in 30 minutes by addition of the indicated concentration of DA. After another 60 minutes in culture the cells were rinsed twice in 2 ml of KRH and removed from the plate for assay of NE (Panel A) and AA (Panel B) as described under Methods. Results are shown from 6 experiments, with an “*” indicating p < 0.05 compared to cells not treated with AA at the same DA concentration by two-way ANOVA. Both concentrations of DA significantly enhanced NE generation compared to cells not treated with DA in the presence or absence of AA (one-way repeated measures ANOVA, Tukey’s post-hoc test, p < 0.05).