Figure 1.

Temperature-dependent electrophoretic mobility of p53(1–93). Shown are gel images obtained by SDS-PAGE performed at (A) 10°C and (B) 40°C. In each gel image, lane 1 contained protein standards with molecular weights in kilodaltons (kDa) as indicated, and lane 2 contained a mixture of purified recombinant human prion protein (22.8 kDa), staphylococcal nuclease (16.8 kDa), and recombinant human lysozyme (14.7 kDa) used as controls. Lane 3 in both images contained purified recombinant p53(1–93) with the N-terminal histidine tag intact, and lane 4 contained purified p53(1–93) after the histidine tag was removed by thrombin digestion.