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A model to explain the L27 mutagenesis effect on tRNA dynamics. The tRNA binding sites and the dipeptide are represented as the same as in Figure 1. In the Rb-WT post-translocation complex, the K4-peptidyl tRNA interaction stabilizes the peptidyl tRNA (the double dashed line). This explains the decreased P/P subpopulation in ribosomes without the K4 residue (Rb-AHK, Rb-9 aa, and Rb-K4E). [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]
