Figure 2.

The PNT domains of PntP2 and Ets1 share similar helical secondary structures. Shown are the predicted helical scores for (A) 2P-Ets1^29–138 (data from Ref. 4), (B) PntP2^142–252, (C) 2P-PntP2^142–252, and (D) 3P-PntP2^142–252, based on an analysis of ¹H^N, ¹⁵N, ¹³C^α, ¹³C^β, and/or ¹³C′ chemical shifts using the program MICS.²⁸ Consideration of phosphorylation-dependent chemical shift changes³² does not significantly alter the scores for the phosphoacceptor serines/threonines (asterisks). The top cartoon and the gray rectangles indicate the observed consensus α-helices (or 3₁₀-helix for H2′) in the NMR-derived structural ensembles of Ets1^29–138 (2JV3.pdb) and 2P-Ets1^29–138 (2KMD.pdb).⁴