Figure 3.

PntP2^142–252 phosphoacceptors and helix H0 are flexible. Steady-state heteronuclear ¹⁵N-NOE values for (A) PntP2^142–252, (B) 2P-PntP2^142–252, and (C) 3P-PntP2^142–252. Well-ordered residues have NOE values of ∼0.8, whereas decreasing values indicate increasing mobility of the ¹⁵N–¹H^N bond vector on the nsec–psec timescale.²⁹ The top cartoon and the gray rectangles indicate the helices, based on the consensus MICS scores, for the three PntP2^142–252 species [H0/H1, 158–175; H2, 187–200; H2′, 209–212; H3, 216–221; H4, 224–230; H5, 234–250; Figs. 2(B–D)], and the phosphothreonines are identified with asterisks. Missing data correspond to prolines or residues with overlapping signals. The error is ∼5%.