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Decay of Luc-SL mRNA is accelerated by HU treatment. ARCA-Luc-SL (A,B) or BTH-Luc-SL (C,D) were introduced into synchronous S-phase HeLa cells by nucleoporation, and the loss of 5′ (A,C) or 3′ (B,D) sequences was followed by qRT-PCR. Some cells were treated with HU immediately after nucleoporation (open symbols).
