FIGURE 1.

IL7R minigene constructs. (Top) IL7R exon 6 (94 nt) and 614 and 573 nt of introns 5 and 6 were cloned into the pI-12 vector. The position of the MS-associated SNP rs6897932 is highlighted with an asterisk (*). U and D represent constitutively spliced upstream and downstream adenoviral exons, respectively. T7 and Sp6 represent sites for common PCR primers that were used to detect products that either included or skipped exon 6. (Bottom) Exon 6 was divided into short blocks that were either mutated or deleted. Larger sequence blocks that were mutated around the SNP are also shown. All substitutions/deletions were introduced in the context of the MS-associated “C” allele. Intronic sequences are shown in lowercase letters; exonic sequences are shown in uppercase letters. Sequences of the substitution mutations are shown below exon 6 sequence.