FIGURE 2.

Identification of the cis-acting regulatory elements within IL7R exon 6. Mutagenesis across exon 6 was performed to identify cis-acting regulatory elements that mediate exon 6 splicing. Control and mutant minigenes were transiently transfected into DT cells and total RNA was analyzed for exon 6 inclusion 48 h post-transfection. Percent exon 6 inclusion was determined from semiquantitative RT-PCRs as a ratio between the “included” and “included + skipped” products and plotted against each mutant. All transfections were performed in triplicate. Error bars represent standard deviation. (A) Gel images representing products of the semiquantitative RT-PCRs for different substitution mutants. (B) Quantification of the RT-PCR results shown in A.