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. 2013 Jan;19(1):103–115. doi: 10.1261/rna.035410.112

FIGURE 5.

FIGURE 5.

Effect of the consensus 5′ splice site on exon 6 inclusion. The exon 6 5′ splice site (AGgugacc, intronic sequences in lowercase) was mutated to match the consensus 5′ splice site sequence (AGgugagu) in the context of minigenes “C”, sub(36–40), CpAmut and sub(36–40)pAmut. The presence of the consensus 5′ splice site resulted in nearly 100% inclusion of exon 6 in all minigene contexts. (A) Representative gel images of the RT-PCR products. (B) Quantification of the RT-PCR results shown in A.