Table 3.
Stress responsive shoot proteins and metabolites of six replicates as fold change.
| Stressed Drought |
Stressed Salt |
Non-stressed Controls |
|||
|---|---|---|---|---|---|
| DN-fed/CN-fed | DN-fix/CN-fix | SN-fed/CN-fed | SN-fix/CN-fix | CN-fed/CN-fix | |
| PROTEINS | |||||
| 1. Photosystem (PS) | |||||
| 1.1 PS.lightreaction | |||||
| G7IJ45 photosystem II 10 kDa polypeptid | ns | ns | ns | 3.8 (0.002) | ns |
| G7JH56 photosystem II CP47 chlorophyll apoprotein | ns | ns | 2.2 (0.005) | ns | ns |
| G7JE46 thylokoid luminal 16.5 kDa protein | ns | ns | ns | 0.4 (0.009) | ns |
| G7JAX6 photosystem I reaction center subunit N | ns | 0.3 (0.029) | ns | ns | 2.9 (0.043) |
| G7JQA7 apocytochrom f | 0.3 (0.0029) | ns | ns | ns | ns |
| B7FIU4 ATP synthase gamma chain | ns | 2.8 (0.003) | ns | ns | 0.5 (0.010) |
| B7FIR4 ATP synthase gamma chain | ns | 0.5 (0.009) | ns | ns | ns |
| G7JAI2 ATP synthase | ns | ns | 3.5 (0.0030) | ns | ns |
| 1.2 PS.photorespiration | |||||
| G7JAR7 serin hydroxymethyltransferase | ns | 0.5 (0.019) | ns | ns | ns |
| 1.3 PS.calvin cycle | |||||
| G7J252 ribulose bisphosphate carboxylase small chain | ns | ns | 3.3 (0.020) | ns | 3.2 (0.005) |
| 2.1.2 Major CHO metabolism.synthesis.starch.transporter | |||||
| G7LDP4 ADP, ATP carrier protein | ns | ns | 2.1 (0.014) | ns | ns |
| 3.4 Minor CHO metabolism.myo-inositol | |||||
| G7J4B5 l-myo-inositol-1 phosphate synthase | 5.3 (0.0001) | ns | ns | ns | 4.0 (0.033) |
| G7LAD5 l-myo-inositol-1 phosphate synthase | 2.0 (0.0204) | ns | ns | ns | ns |
| 6.3 Gluconeogenesis.Malate DH | |||||
| G7JTZ0 Malate dehydrogenase | ns | ns | ns | 0.4 (0.000) | ns |
| 7.1 OPP.oxidative PP.6-phosphogluconate dehydrogenase | |||||
| Q2HVD9 6-phosphogluconate dehydrogenase | ns | 0.5 (0.001) | ns | ns | 2.0 (0.001) |
| 9.9 Mitochondrial electron transport/ATP synthesis.F1-ATPase | |||||
| G7LCJ4 ATP synthase delta subunit | 2.5 (0.0001) | ns | ns | ns | 2.2 (0.048) |
| 10.1 Cell wall.precursor synthesis | |||||
| G7L571 UDP-glucose 6-dehydrogenase | ns | 0.3 (0.001) | ns | ns | ns |
| 11.1 Lipid metabolism.FA synthesis and FA elongation | |||||
| G7LIV6 biotin carboxylase | ns | 0.4 (0.032) | ns | ns | 2.6 (0.023) |
| G7JNN1 Acyl-[acyl-carrier-protein] desaturase | ns | ns | 3.6 (0.014) | ns | ns |
| 11.6 Lipid metabolism.lipid-transfer proteins | |||||
| G7JID0 non-specific lipid-transfer protein | 2.5 (0.002) | ns | ns | ns | ns |
| 12.2 N-metabolism.ammonia metabolism.glutamate synthase | |||||
| Q2HW53 ferredoxin-dependent glutamate synthase | ns | 0.5 (0.000) | ns | ns | ns |
| P04078 glutamine synthetase cytosolic isozyme | ns | ns | ns | 0.5 (0.004) | 3.8 (0.004) |
| 13.1 Amino acid metabolism.synthesis | |||||
| Q6J9 × 6 SAMS | 2.2 (0.0079) | ns | ns | ns | ns |
| A4ULF8 SAMS | 2.4 (0.0007) | ns | ns | ns | ns |
| A4PU48 SAMS | ns | 0.5 (0.009) | ns | ns | ns |
| G7L3W1 SAMS | ns | 0.5 (0.002) | ns | ns | ns |
| G7JTY4 LL-diaminopimelate aminotransferase | ns | ns | 0.4 (0.021) | ns | ns |
| G7J013 alanine glyoxylate aminotransferase | ns | ns | ns | 2.4 (0.005) | ns |
| 15.2 Metal handling.binding, chelation, and storage | |||||
| G7K283 ferritin | ns | ns | 4.0 (0.018) | ns | ns |
| G7JLS7 ferritin | 11.4 (0.005) | ns | 10.0 (0.0004) | ns | ns |
| 16.2 Secondary metabolism.phenylpropanoids | |||||
| G7JTH6 caffeic acid 3-O-methyltransferase | 6.5 (0.0000) | ns | 4.0 (0.0023) | ns | ns |
| 19.10 Tetrapyrrole synthesis | |||||
| G7IK85 Mg-chelatase subunit chlI | 0.3 (0.0002) | 0.3 (0.0005) | ns | 0.5 (0.004) | ns |
| 20.1 Stress.biotic | |||||
| B0RZH7 putative thaumatin-like protein | ns | 0.4 (0.000) | ns | ns | 2.2 (0.001) |
| G7IYL0 receptor-like protein kinase | ns | 0.5 (0.002) | ns | ns | ns |
| 20.2 Stress.abiotic | |||||
| Q2HT97 heat shock protein Hsp70 | ns | ns | ns | 0.5 (0.034) | ns |
| G7JGC6 low-temperature inducible | 2.3 (0.0001) | ns | ns | ns | 2.3 (0.001) |
| G7JGC9 low-temperature inducible | ns | ns | ns | 0.3 (0.021) | ns |
| 21.5 Redox.peroxiredoxin | |||||
| G7JS60 peroxiredoxin Q | 2.6 (0.0000) | ns | ns | ns | ns |
| 23.4 Nucleotide metabolism.phosphotransfer and pyrophosphatases | |||||
| G7JMM2 nucleoside diphosphate kinase | ns | ns | 2.0 (0.040) | ns | ns |
| B7FIM7 soluble inorganic pyrophosphatase | ns | 03 (0.006) | ns | ns | ns |
| 26.20 Misc.ferredoxin-like | |||||
| G7KWY5 ferredoxin | ns | 0.3 (0.011) | ns | ns | 2.1 (0.037) |
| 26.4 Misc.beta-1,3 glucan hydrolases | |||||
| G7JQL4 endo-beta-1,3-glucanase | ns | ns | 0.5 (0.014) | ns | ns |
| 27.1 RNA.processing | |||||
| G7JK09 Poly(A)-binding protein | ns | 0.4 (0.000) | ns | ns | ns |
| 27.4 RNA.RNA binding | |||||
| G7JG67 glycerine-rich RNA binding protein | 0.5 (0.0059) | ns | ns | ns | ns |
| 29.2 Protein.synthesis | |||||
| Q945F4 eukaryotic translation initiation factor 5A-2 | ns | 0.4 (0.003) | ns | ns | 2.5(0.003) |
| G7IH13 elongation factor EF-2 | ns | ns | ns | 2.5 (0.000) | ns |
| 29.5 Protein.degradation | |||||
| G7LIT0 ATP-dependent Clp protease | 0.4 (0.0226) | ns | ns | ns | ns |
| G7ZVC0 presequence protease | ns | 0.5 (0.010) | ns | ns | ns |
| G7K8J5 bi-ubiquitin | ns | ns | 0.3 (0.024) | ns | ns |
| G7LB82 proteasome subunit alpha type | ns | ns | 2.1 (0.019) | ns | ns |
| 31.1 ′Cell.organization | |||||
| G7IAN2 tubulin ß chain | ns | ns | 5.4 (0.0006) | ns | ns |
| G7L5V0 tubulin ß chain | ns | ns | 3.0 (0.0205) | 0.4 (0.046) | ns |
| G7KB73 annexin | 2.0 (0.0394) | ns | ns | ns | 0.5 (0.000) |
| G7JAX5 actin | ns | ns | 3.8 (0.0001) | ns | ns |
| 34.1 Transport. p- and v-ATPases | |||||
| A6Y950 Vacuolar H + -ATPase B subunit | ns | 0.5 (0.001) | ns | ns | ns |
| “PUTATIVE” UNCHARACTERIZED PROTEINS | |||||
| B7FJY9 similar 94.0% Q9SQL2, CB24_PEA, chlorophyll a-b binding protein P4, chloropl., Pisum sativum (garden pea), e = 1.0 × 10−178 | 3.0 (0.0001) | ns | ns | ns | ns |
| B7FMC4 similar 73.0% Q03666, GSTX4_TOBAC, probable glutathione S-transferase, Nicotiana tabacum (common tobacco), e = 1.0 × 10−121 | 2.1 (0.007) | ns | ns | ns | 2.6 (0.036) |
| B7FJR8 similar 83.0% Q9LZG0, ADK2_ARATH, adenosine kinase 2, Arabidopsis thaliana (mouse-ear cress), e = 0 | ns | 0.4 (0.000) | ns | ns | 2.1 (0.015) |
| B7FM78 similar 97.0% P81406, GAPN_PEA, NADP-dependent glyceraldehyde-3-phosphate dehydrogenase, Pisum sativum (garden pea), e = 0 | ns | 0.5 (0.023) | ns | ns | ns |
| B7FKR5 similar 99.0% O24076, GBLP_MEDSA, guanine nucleotide-binding protein subunit beta, Medicago sativa (alfalfa), e = 0 | ns | 0.4 (0.005) | ns | ns | 2.6 (0.001) |
| B7FI14 similar 64.0% Q9LEH3, PER15_IPOBA, peroxidase 15, Ipomea batatas (sweet potato) (Convolvulus batatas), e = 1.0 × 10−132 | ns | 0.4 (0.000) | ns | ns | 2.2 (0.001) |
| B7FL15 similar 85.0% P13443, DHGY_CUCSA, glycerate dehydrogenase, Cucumis sativus (cucumber), e = 2.0 × 10−71 | ns | 0.3 (0.000) | ns | ns | 2.7 (0.000) |
| G7I4F9 uncharacterized protein | ns | 0.4 (0.021) | ns | ns | 2.1 (0.035) |
| B7FHX0 similar 98.0% P29500, TBB1_PEA, tubulin beta-1 chain, Pisum sativum (garden pea), e = 0 | ns | ns | 4.4 (0.0035) | ns | 2.2 (0.002) |
| B7ZWQ5 similar 90.0% Q40977, MDAR_PEA, monodehydroascorbate reductase, Pisum sativum (garden pea), e = 0 | ns | ns | 2.0 (0.035) | ns | ns |
| B7FL16 similar 84.0% P13443, DHGY_CUCSA, glycerate dehydrogenase, Cucumis sativus (Cucumber), e = 2.0 × 10−88 | ns | ns | ns | 0.5 (0.043) | ns |
| B7FI41 similar 52.0% Q41160, LCB3_ROBPS, putative bark agglutinin LECRPA3, Robinia pseudoacacia (BLAQCK locust), e = 5.0 × 10−87 | ns | ns | ns | 0.4 (0.023) | ns |
| G7KAG7 similar 71.0% Q9THX6, TL29_SOLLC, thylakoid lumenal 29 kDa protein, chloroplast, Solanum lycopersicum (tomato; Lycopersicon esculentum), e = 1.0 × 10−172 | ns | ns | ns | 0.5 (0.032) | ns |
| B7FNH1 similar 79.0% O23755, EF2_BETVU, elongation factor 2, Beta vulgaris (sugar beet), e = 2.0 × 10−67 | ns | ns | ns | 0.3 (0.004) | 3.6 (0.004) |
| METABOLITES | |||||
| Major CHO metabolism | |||||
| Glucose | ns | 10 (0.034) | ns | 0.3 (0.009) | 0.5 (0.014) |
| Glucose-1-p | ns | ns | ns | 5.1 (0.000) | 0.5 (0.014) |
| Maltose | ns | ns | ns | 2.3 (0.003) | ns |
| Ribitol | 3.2 (0.010) | ns | ns | ns | ns |
| Amino acid metabolism | |||||
| Glutamate | ns | ns | 2.1 (0.010) | ns | ns |
| Leucine | ns | 6.1 (0.000) | 2.7 (0.006) | 2.2 (0.012) | ns |
| Proline | 0.5 (0.049) | ns | 2.6 (0.006) | ns | ns |
| Valine | ns | ns | 2.4 (0.000) | 2.4 (0.012) | ns |
| Aspartate | ns | 0.3 (0.021) | ns | ns | ns |
| TCA | |||||
| 2-Oxoglutarate | ns | ns | ns | 0.3 (0.040) | ns |
| Citrate | ns | ns | 0.5 (0.008) | 0.3 (0.000) | ns |
| Succinate | ns | ns | ns | 0.5 (0.008) | ns |
| Malate | ns | ns | ns | 0.2 (0.001) | 2.0 (0.012) |
| Malonate | ns | 0.5 (0.029) | 0.4 (0.000) | 0.1 (0.001) | 2.1 (0.001) |
| Others | |||||
| Phosphate | ns | ns | 0.5 (0.006) | 0.2 (0.000) | ns |
(Student’s t test p < 0.05 in brackets and fold change ≥ 2; n = 6) with significantly altered abundances in response to spectral counts of stress proteins and peak area of metabolites (IS and DW normalized). Protein category headers including binning numbers of the MapMan mapping file. CN-fed, control, N-fertilized; CN-fix, control, N-fixation; DN-fed, drought, N-fertilized; DN-fix, drought, N-fixation; SN-fed, salt, N-fertilized; SN-fix, salt, N-fixation; numbers 1 – 6 indicate replicates. ns, not significant; SAMS, S-adenosylmethionone synthetase.