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. 2012 Sep 12;97(1):33–43. doi: 10.1093/cvr/cvs287

Figure 3.

Figure 3

Involvement of Akt (A–C) and PKA (D–F) in early IPC. Representative immunoblots of hearts subjected to control perfusion (Con), IPC, Con/R15min and IPC/R15min for pAktSer473 (pAkt) and Akt (A) with quantitative bar graphs of pAkt and Akt in (B). (C) First column phase contrast images (PhC) and second column immunostaining of pAkt (green fluorescence) in hearts subjected to control perfusion and IPC treatment. Representative immunoblots of hearts subjected to Con, IPC, Con/R15min, and IPC/R15min for pPKAThr197 (pPKA) and PKA (D) with quantitative bar graphs of pPKA and PKA in (E). (F) First column phase contrast images (PhC) and second column immunostaining of pPKA (green fluorescence) in hearts subjected to control perfusion and IPC treatment. Values are means ± SE. n = 4/group **P < 0.01 vs. control hearts; †P < 0.05 vs. Con/R15min group.