Figure 6.

The activation of selected mitogen-activated protein kinases involved in cardiac hypertrophy: (A) levels of phospho-ERK1/2 (upper blot), total ERK1/2 (middle blot), and the corresponding blot for α-tubulin (lower blot) in the experimental group. Corresponding quantitative data are shown in (B). As shown phospho-ERK1/2 levels were reduced in the Myoz2^+/−:MYOZ2^WT as opposed to the corresponding levels in the NTG mice or the Myoz2^+/−:MYOZ2^I246M mice. (C) Levels of phospho-p38 (upper blot), total p38 (middle blot), and α-tubulin in the experimental groups. There were no significant differences in the ratio of phosphor-p38/total p38 among the experimental groups (D). (E) Levels of phospho-JNK54/46 (upper blot), total JNK54/46 (middle blot), and the corresponding α-tubulin levels (lower blot). A shown (F) relative levels of phospho-JNK54/46 normalized to total JNK54/46 levels were significantly reduced in Myoz2^+/−:MYOZ2^WT and Myoz2^+/−:MYOZ2^I246M mice when compared with levels in the NTG mice. However, there were no significant differences between the wild-type and mutant bigenic mice groups.