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. Author manuscript; available in PMC: 2013 Dec 13.
Published in final edited form as: Cell Host Microbe. 2012 Dec 13;12(6):806–814. doi: 10.1016/j.chom.2012.10.013

Figure 1. Identification of GFP-Us9 interacting proteins.

Figure 1

(A) Schematic diagram for parallel IPs of GFP (PRV 151) and GFP-Us9 (PRV 340) from PRV infected PC12 cells. (B) Representative Coomassie-stained gel of a GFP-Us9 IP (4–12% polyacrylamide gel). (C) Western blot analysis of GFP and GFP-Us9 IP samples with anti-GFP and anti-Us9 antibodies (10% polyacrylamide gel). (D) Visualization of putative Us9-associated proteins at 20 hpi. Proteins were manually categorized based on their biological function or subcellular localization and color-coded according to their enrichment value. Proteins highlighted with blue borders were depleted or not detected in at least one of the functionally defective GFP-Us9 IP samples compared to wild type GFP-Us9 as presented in Figure S2. See also Figure S1 and Table S1.