Figure 2.
Biological meaning of weak TFE3 nuclear staining. (A), (B) an example of Strong TFE3 nuclear staining at low and high power, respectively. (C), (D) an example of weak nuclear TFE3 staining at low and high power, respectively. (E) RT-PCR results from a weak nuclear TFE3 staining case using primers for wild-type (non-translocation) and all 5 known translocation types. Only wild-type (non-translocation) TFE3 transcript was detected. (F) RT-PCR Beta-actin control results from the same sample. (G) Example of FISH results from weak TFE3 nuclear staining cases. There was no evidence of TFE3 gene rearrangement in these cases.