FIGURE 7.

The diagram of Pih1 regions and motifs that are involved in R2TP complex formation. Pih1 is represented by a rectangle from amino acid 1 to amino acid 344. The Pih1 fragments used in this study were color coded as Pih1^1–230 (blue), Pih1^231–284 (orange), and Pih1^285–344 (yellow). It should be noted that fusion protein GFP-Pih1^282–344 used in this study contains Pih1 fragment Pih1^282–344 that starts two amino acids before Leu²⁸⁴. The two predicted intrinsically disordered regions IDR1 (Gly¹⁹⁵–Leu²²⁷) and IDR2 (Asn²⁴⁰–Tyr²⁶⁷) are also highlighted. The five hydrophobic amino acids clusters in the Pih1 C terminus are indicated as gray bars, and the E236 is indicated as black bar. The overall sequence fragments required for Rvb1/Rvb2 and Tah1 bindings are indicated on the top of the Pih1.