FIGURE 8.

Leakage of phospholipid vesicles treated with preaggregated Tau protein. Large unilamellar vesicles filled with the fluorescence dye ANTS and the quencher DPX were treated for 220 min with Tau protein preaggregated for 0, 24, or 144 h. During the time of treatment, the fluorescence yield of ANTS was measured. ANTS fluorescence increases upon separation from DPX, which is caused by vesicle leakage. Tau aggregated for 24 h induces the greatest vesicle leakage compared with long-aggregated and non-aggregated Tau.