TABLE 1.
MIC data, steady-state kinetic parameters, and dissociation constants for AAC(6′)-Ie/APH(2″)-Ia
| Aminoglycoside | MICa | kcat | Km | kcat/Km | Kib |
|---|---|---|---|---|---|
| μg/ml | s−1 | μm | m−1 s−1 | μm | |
| 4,6-Disubstituted | |||||
| Kanamycin A | 512/4 | 0.57 ± 0.02 | <1 | >5.7 × 105 | |
| Kanamycin B | 64/1 | 0.12 ± 0.01 | <1 | >1.2 × 105 | |
| Tobramycin | 64/0.5 | 0.11 ± 0.01 | <1 | >1.1 × 105 | |
| Dibekacin | 32/0.5 | 0.15 ± 0.01 | 0.3 ± 0.2 | (5 ± 4) × 105 | |
| Arbekacin | 0.5/0.25 | 2.3 ± 0.1 | 0.8 ± 0.2 | (2.9 ± 0.7) × 106 | |
| Amikacin | 4/2 | 3.7 ± 0.1 | 33 ± 4 | (1.1 ± 0.1) × 105 | |
| Gentamicin C | 32/0.25 | 3.0 ± 0.1 | 0.6 ± 0.3 | (5 ± 2) × 106 | |
| Sisomicin | 16/0.25 | 8.1 ± 0.2 | 0.8 ± 0.2 | (1.0 ± 0.2) × 107 | |
| Netilmicin | 2/0.25 | 6.5 ± 0.1 | 0.41 ± 0.05 | (1.6 ± 0.2) × 107 | |
| 4,5-Disubstituted | |||||
| Neomycin B | 1/0.5 | 0.26 ± 0.02 | |||
| Paromomycin | 4/4 | 5.3 ± 0.3 | |||
| Lividomycin A | 8/4 | 11 ± 1 | |||
| Butirosin A and B | 140 ± 10 | ||||
| Atypical | |||||
| Neamine | 16/8 | 0.10 ± 0.01 | |||
a Ratio of the MICs with E. coli JM83 harboring pBluescript::AAC(6′)-Ie/APH(2″)-Ia to the E. coli JM83 host strain.
b Dissociation constant for the binary enzyme·GTP complex.