FIGURE 3.

HPAEC-PAD chromatograms of donor/acceptor specificity experiments using starch, isomaltose (IM), and glucose. A, starch blank (0.4%, w/v; solid line) and following incubation with CjAgd31B (dotted line) showing a reduction of short malto-oligosaccharides (M to M₅) and an increased amount of medium length malto-oligosaccharides (M₇ to M₁₂). B, starch (0.4%) following incubation with CjAgd31B with no additional acceptor substrate added (solid line), with added isomaltose (1 mm; dashed line), and with added glucose (1 mm; dotted line). With both alternate acceptor substrates, there is a clear increase in the amount of short to medium length oligosaccharides formed compared with starch alone. nC, nanocoulomb.