FIGURE 5.

In trans complementation of ΔaapFΔflaX (MW452) strain. A, schematic representation of different flaX truncates that were tested for their ability to complement a ΔflaX strain. B, expression levels of the truncates in S. acidocaldarius. C, soft gelrite motility assay of complemented strains. As control experiment MW351 with pSVA1614 was used as positive control and MW456 with pSVA352 (a E to A mutant of the Walker B box in FlaI on pSVA1450 backbone (S. Reindl, A. Ghosh et. al., unpublished data) was used as negative control. D, transmission electron microscopic images of flaX and flaXC1 expressing MW452 strains, respectively.