FIGURE 1.

Wound healing ability of wild-type and mutant CHO cells. A, immunofluorescence. Wild-type CHO cells, paclitaxel-resistant (and dependent) mutant Tax 11-6, and colcemid-resistant mutant CV 2-8 were stained with an antibody to α-tubulin and with DAPI to compare their microtubule cytoskeletons and cell morphologies. Scale bar, 10 μm. B, wound healing assay. Nearly confluent cultures of each of the three cell lines were scratched to form cell-free zones, which were photographed with a 4× objective at various times to measure how quickly the wound closed. Time points at 0 and 7 h are shown. Scale bar, 100 μm.