FIGURE 1.

SMC2 is up-regulated in human CRC. A, WB analysis of SMC2 in human CRC. A representative subset of 29 cases studied is shown. Actin was used as loading control. B, quantitative real-time PCR for SMC2 in 16 pairs of colon adenocarcinoma tumors and matched adjacent normal colonic tissues. Data are representative of three independent experiments. The mean values of SMC2 levels were compared using Student's t test (upper boxplot). C and D, SMC2, SMC4, and β-catenin levels were evaluated by WB in both colorectal cancer cell lines (n = 14) and samples from CRC patients (n = 27, a representative subset is shown). Actin was used as loading control. E and F, SMC2, SMC4, and β-catenin protein levels on WB were determined by gel band quantification and normalized to the corresponding actin levels. Values were used to perform correlation studies following Spearman test. G, immunohistochemistry of SMC2 in paraffin-embedded tissue. A representative specimen is shown. Magnified regions of the normal and tumor mucosa are shown on the right. N, normal tissue; T: tumor tissue (adenocarcinoma).