FIGURE 4.

Determination of the minimal regulatory region of SMC2 promoter. A, relative position and sequences of the putative TBEs predicted in silico in the SMC2 promoter and deletion mutants for luciferase (luc) reporters performed. B, determination of fragment 3 as the minimal regulatory region of the SMC2 promoter. Luciferase activity of each deletion mutant was normalized to Renilla luciferase internal control (RLU, relative luciferase units) in DLD1 (left) or HCT116 (right) cell lines; a representative result is shown of at least three independent experiments. *, p < 0.05; **, p < 0.01; Student's t test (promoter activity versus full-length SMC2 promoter (SMC2 FL).