Skip to main content
. 2012 Oct 26;287(52):43492–43501. doi: 10.1074/jbc.M112.394023

FIGURE 1.

FIGURE 1.

Expression, autocleavage, and secretion of CHRD module deletants. A, schematic representation of V5-tagged WT human PCSK9, PCSK9 domain deletants (L455X, lacking CHRD, and CHRD, lacking the prosegment (PRO) and catalytic domain but retaining the hinge region (10)), and PCSK9 CHRD module deletants (ΔM1, ΔM2, ΔM3, ΔM12, ΔM23, and ΔM13). SP: Signal Peptide. B, HEK293 cells were transiently transfected with cDNAs encoding the above constructs. 48 h post-transfection, proteins in cell lysates and media were resolved by SDS-PAGE and analyzed by Western blot analysis using mAb-V5. The migration positions of reference molecular weight proteins, as well as those of the precursor and mature PCSK9 products are emphasized. The secreted CHRD module deletants are labeled with a star. Ctl, control.