FIGURE 2.

Two-dimensional BN/SDS-PAGE and Western blot analysis of WT Synechocystis sp. PCC 6803 Hox hydrogenase. A, the soluble fraction of WT Synechocystis sp. PCC 6803 was separated on a 12% (w/v) polyacrylamide BN-polyacrylamide gel followed by two-dimensional separation on a 17.5% (w/v) polyacrylamide SDS-polyacrylamide gel (B) and analysis by Western blotting with the indicated Hox subunit-specific antibodies (C–G). The first dimension BN-polyacrylamide gel was Coomassie-stained, whereas the second dimension SDS-polyacrylamide gel was silver-stained.