FIGURE 5.

His₆ pull-down of Synechocystis sp. PCC 6803 Hox subcomplexes. hox⁻ mutant expressing His₆-HoxYH (A), hoxH⁻ mutant expressing His₆-HoxH (B), and hoxE⁻ mutant expressing His₆-HoxE (C) were used for pull-down on Co²⁺ beads to determine subunit association. Supernatant, flow-through (FT) following bead incubation, pooled washes with/without 0.5 m NaCl, and boiled bead samples from each pull-down were run on TGX Any kDa gradient SDS-polyacrylamide gels (Bio-Rad), transferred to PVDF, and immunoblotted using Hox subunit-specific antibodies.