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. 2012 Nov 6;287(52):43527–43532. doi: 10.1074/jbc.M112.401141

FIGURE 3.

FIGURE 3.

The ALC1 macrodomain protects PAR chains from ∼ 3 to more than 20 ADP-ribose units from digestion by PARG. A, diagram showing the protocol used for PAR footprinting assays. B, reactions performed as diagrammed in A contained no PARG (lanes 1 and 5) or 1.5 ng (lanes 2 and 6), 5 ng (lanes 3 and 7), and 15 ng (lanes 4 and 8) PARG. C, reactions performed as diagrammed in A contained PARP1 and wild-type or mutant ALC1 without or with 5 ng of PARG. Marker lanes (M) show the total reaction products synthesized in a reaction containing nucleosomes and PARP1. Free ATP runs at the position indicated by the asterisk.