TABLE 2.
Growth of wild-type and sec12 mutant yeast
| SEC12a | Growthb |
|---|---|
| Wild-type | + |
| pRS415c | − |
| N40A | − |
| I38D | − |
| I38A | +/− |
| Y15K | + |
| N35A | + |
| N36A | + |
| N36Q | + |
| K41A | + |
| S72A | + |
| Y132D | + |
| E175K | + |
| D287K | + |
a Haploid SEC12Δ S. cerevisiae carrying pRS416-SEC12 was transformed with pRS415-SEC12 plasmids and then plated on 5-fluoroorotic acid to select against pRS416-SEC12.
b +, growth similar to wild-type; −, no growth (at 30 °C). I38A (+/−) displayed a significant growth defect at 30 °C, which was markedly exacerbated at 37 °C.
c pRS415 plasmid lacking SEC12.