FIGURE 2.

Increased diffusion of rΔF508CFTR in MDCK cell lines and transiently transfected COS-7 cells. A, low temperature corrected ΔF508CFTR surface density (left) and endocytic rates (right) in MDCK cell lines immediately after rescue (red) and after 1 h 37 °C incubation (blue). B, example trajectories of wtCFTR (black), rΔF508₂₇, (red), and rΔF508₃₇, (blue) diffusion in MDCK cell lines. C, cumulative distribution curves for MDCK cell lines expressing wtCFTR (black), rΔF508₃₇ (blue), or CFTRΔPDZ (pink) and co-expressing mCh-CFTR-C with wtCFTR (gray) or rΔF508₃₇, (green). D, summary graph representing median (blue lines) and interquartile (red lines) range values for the diffusion of wtCFTR, rΔF508CFTR and CFTRΔPDZ-expressing MDCK cell lines. As indicated, mCh-CFTR-C was also expressed (bars 4 and 5). E, surface levels (left) and endocytic rates (right) of low temperature corrected 3FLAG-tagged ΔF508CFTR in transiently transfected COS-7 cells immediately after rescue (red) and after 37 °C incubation (blue). F, summary graphs of diffusive range for 3FLAG-tagged wtCFTR, ΔF508CFTR, CFTRΔPDZ, and ΔF508CFTRΔPDZ expressing COS-7 cells. In panels D and F, statistically significant differences in populations are shown (*, p < 0.001). SPT data for MDCK cells was derived from 113–788 trajectories and for COS-7 cells from 140–252 trajectories. Data for CFTR surface density and endocytic rates are mean ± S.E. for 3–8 independent experiments.