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. 2012 Oct 31;287(52):43639–43650. doi: 10.1074/jbc.M112.393678

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Evidence that the HNS-RRM3 motif is involved in the recognition and degradation of HuR by β-TrCP1. A, upper panel, schematic representation of the structures of wild-type and various truncated mutant forms of HuR-FLAG. Lower panel, coimmunoprecipitation analysis of the interaction of wild-type HuR versus various truncated HuR mutants with β-TrCP1. LNCaP cells were transiently cotransfected with Myc-tagged β-TrCP1 and FLAG-tagged wild-type HuR or individual truncated mutants for 48 h. Immunoprecipitation (IP) with anti-FLAG-agarose conjugates and immunoblotting with anti-Myc and anti-FLAG antibodies were performed as described in Fig. 6. B, time-dependent effect of CG-5 on the degradation of FLAG-tagged HuR and various truncated HuR mutants versus endogenous HuR.