FIGURE 5.

Inhibition of MMP-2 and TACE activity by released TIMP-3. A, dose-dependent displacement of recombinant TIMP-3 (25 nm) binding on heparin by N6L using ELISA test. B and C, MDA-MB-435 cells were treated or not with 10 μm N6L for 48 h in FBS-free medium. B, conditioned media were removed and added to recombinant MMP-2 protein. MMP-2 activity was measured by fluorometry using fluorescent peptidic substrate. C, inhibition of TNFR1 cleavage by TACE after N6L treatment. Lysates of nontreated (Control) or N6L treated cells were analyzed by Western blotting.