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. 2012 Oct 30;287(52):43741–43752. doi: 10.1074/jbc.M112.372318

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — Usp9x interacts with the SMN complex. A, diagram of the FLAG-Usp9x-HTBH expression construct. B, immunoblotting (IB) of FLAG or HRP-conjugated streptavidin in cell lysates from 293T cells stably expressing FLAG-HTBH (control) or FLAG-Usp9x-HTBH. C, silver staining of purified Usp9x and its interacting proteins resolved by SDS-PAGE. D, peptides of SMN, Gemins 2–5 identified by mass spectrometry. E, immunoblotting of SMN, Usp9x, and Gemins in mass spectrometric samples shown in C. F, immunoblotting of proteins co-immunoprecipitated with endogenous SMN in HeLa cells. G, immunoblotting of proteins co-immunoprecipitated with endogenous Usp9x in HeLa cells.