Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Oct 30;287(52):43741–43752. doi: 10.1074/jbc.M112.372318

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 6. — Usp9x does not regulate SMNΔ7 ubiquitination and degradation. A, immunoblotting (IB) of HA-Ub conjugated on FLAG-SMNΔ7 in 293T cells. FLAG-SMNΔ7 and HA-Ub were expressed in 293T cells. In A–C, FLAG-SMNΔ7 was immunoprecipitated (IP) by denaturing IP. B, FLAG-SMNΔ7 was immunoprecipitated as shown in A and eluted with 6 m urea plus 2.5% SDS. C, immunoblotting of Myc-Ub conjugated on FLAG-SMNΔ7 in 293T cells overexpressing the indicated proteins. D, immunoblotting of HA-SMNΔ7, Usp9x, and β-actin in cell lysates prepared from control (GFP) or Usp9x stable knockdown HeLa cells that stably express HA-SMNΔ7. Cells were harvested at the indicated time points after treating with 75 μg/ml of CHX. Error bars in the right panel represent S.D. of three independent experiments. E, confocal images of immunofluorescence staining of overexpressed FLAG-SMNΔ7 in HeLa cells.