FIGURE 1.

Effect of pro- and mature NGF on the sortilin-p75^NTR interaction. Representative (n = 4) Western blot (WB) of lysates of HEK293 cells stably expressing either sortilin_mut or sortilin_mut and p75^NTR incubated with 25 nm proNGF or NGF as indicated. The cells were cross-linked with dithiobis succinimidylpropionate, and the lysates were immunoprecipitated (IP) with anti-p75^NTR antibodies prior to SDS-PAGE and Western blotting. Sortilin is co-immunoprecipitated by p75^NTR, and the interaction is stabilized by proNGF but not NGF.