FIGURE 4.

Advanced prostate cancer cells require AIF for survival under growth stress, invasion, and tumorigenesis in vivo. A, LNCaP, DU145, and PC3-derived cells were harvested, washed, suspended in fresh medium, and plated on Matrigel^TM layers at a density of 10,000 cells/well. The cells were then allowed to grow for 4 days before imaging. B, PC3-derived cells were harvested, washed, and suspended in serum-free medium. Cell suspensions were added to BD-BioCoat Matrigel^TM invasion chamber or control chambers and then placed into 12-well plates containing medium with serum. The cells were allowed to invade Matrigel^TM layers for the indicated periods of time at which point chamber membranes were collected, the attached cells were fixed, and the total cells per membrane were counted. The percentage of invasion was determined by comparing cells on Matrigel^TM-coated membranes versus uncoated control membranes. C, PC3-derived cells were injected subcutaneously into the dorsal hind flanks of male athymic nude mice. The mice were palpated daily until tumor growth was established, starting day 0 once tumors reached a size of 50 mm³.