FIGURE 6.

Altered lactate secretion and oxygen consumption following ablation of AIF from advanced prostate cancer cells. A, the indicated cell lines were harvested, washed, and seeded at equal densities in fresh medium. 48 h after plating, medium was collected, and secreted lactate was measured using the Sigma lactate assay kit. The total number of cells in each well was assessed by Coulter counting and used to determine lactate secretion per cell. B, the indicated cell lines were harvested, washed, and seeded at equal densities in fresh medium. MitoXpress® probe was added, and then each well was overlaid with mineral oil to avoid interference from atmospheric oxygen. Fluorescence of each well was then measured every 1.5 min for a total of 200 min.