TABLE 1.
Summary of expressions, localizations and efflux activities of sea urchin ABC transporters
+++, high level protein expression; ++, medium level protein expression; +, low level protein expression.
| Gene (GenBank ID) | Tag position | Expression level | Localization | Efflux activity |
Addgene ID | |||
|---|---|---|---|---|---|---|---|---|
| CAM | b-VIN | b-VER | MX | |||||
| Sp-ABCB1a (JQ390048) | C-mCherry | + | Apical | Yes | ||||
| N-mCherry | +++ | Apical | Yes | Yes | Yes | 34939 | ||
| N-Cerulean | ++ | Apical | No | 34944 | ||||
| No tag | ++ | Yes | 38036 | |||||
| Sp- ABCB4a (JQ354983) | C-mCherry | + | Apical | Yes | ||||
| N-mCherry | +++ | Apical | Yes | Yes | Yes | 34941 | ||
| N-Cerulean | ++ | Apical | No | 34945 | ||||
| Sp- ABCC1 (JQ354984) | C-mCherry | ++ | Basolateral | No | 40587 | |||
| N-mCherry | + | Small apical vesicles | No | 34942 | ||||
| Sp-ABCC5a (JQ354989) | C-mCherry | + | Basolateral | No | ||||
| N-mCherry | ++ | Basolateral | No | 35207 | ||||
| Sp-ABCC9a (JQ355003) | C-mCherry | + | Large apical vesicles | No | 35208 | |||
| N-mCherry | ++ | Toxic, retained in ERa | ||||||
| Sp- ABCG2a (JQ355004) | C-mCherry | +++ | Apical | No | No | No | 34943 | |
| N-mCherry | ++ | Apical | No | No | No | |||
| C-Cerulean | +++ | Apical | Yes | 34946 | ||||
a ER, endoplasmic reticulum.