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. 2012 Oct 26;287(52):43910–43908. doi: 10.1074/jbc.M112.417899

FIGURE 5.

FIGURE 5.

CHMP5(139–195) binds tightly to the LIP5(MIT)2 domain. Fluorescence anisotropy binding isotherms show the binding of fluorescently labeled CHMP5(139–191)(A191C) to a series of different LIP5 constructs (see inset for key). Plotted points are averages of at least four independent anisotropy measurements for each LIP5 concentration, and error bars show S.D. in the measurements. Curves show fits of these isotherms to simple 1:1 binding models. Reported KD values are means ± S.D. from the fits to at least four independent binding isotherms.