TABLE 1.
Biophysical properties of hKv2.1 and hKv2.1/hKv8.2 channels expressed in HEK293 cells
Kinetic parameters for activation (Vn, ½, an) and inactivation (Vh, ½, ah) are given. Time constant of activation and inactivation (τact and τinact) is shown.
| Biophysical property | hKv2.1 | hKv2.1/hKv8.2 |
|---|---|---|
| Activation | ||
| Current density (nA/pF) | 2.04 ± 0.24 (n = 20) | 0.32 ± 0.06 (n = 17)a |
| Vn, ½ (mV) | −14.6 ± 3.1 (n = 8) | −9.5 ± 1.6 (n = 6) |
| an (mV) | 8.8 ± 0.9 (n = 8) | 13.9 ± 1.0 (n = 6)a |
| τact at −20 mV (ms) | 60.0 ± 6.2 (n = 6) | 40.6 ± 3.7 (n = 6)a |
| 10-s depolarization to +30 mV | ||
| % inactivation | 90.2 ± 2.1 (n = 7) | 70.3 ± 5.1 (n = 8)a |
| τinact (s) | 2.8 ± 0.2 (n = 7) | 3.7 ± 0.3 (n = 8)a |
| Steady-state inactivation | ||
| Vh, ½ (mV) | −46.8 ± 1.3 (n = 9) | −47.3 ± 1.5 (n = 7) |
| ah (mV) | 6.5 ± 0.3 (n = 9) | 5.1 ± 0.5 (n = 7)a |
a Significantly different properties (p < 0.05).