FIGURE 6.

Effects of C70A mutation on AnkG dynamics at the lateral membrane. A, 300 ng of WT, C70A, DAR999AAA, and C70A/DAR999AAA double mutant AnkG-GFP were transfected into MDCK cells plated in 14-mm MatTek plates. FRAP was performed using an LSM780 laser-scanning confocal microscope. The representative graphs show the fluorescence recovery within 5 min; yellow boxes indicate the regions bleached. B, quantitative results of n = 10 repetitive measurements. The normalized fluorescence intensities were plotted against time and regressed against the one-phase exponential equation, y = y₀ + Ae⁻k × t, using GraphPad Prism version 5 (scale bar, 10 μm). Error bars, S.E.