Figure 1.

H1299 cells expressing gain-of-function mutant p53 up-regulate protein receptor tyrosine kinase Axl. (A, C) RT-qPCR analysis of Axl levels in H1299 cells stably transfected with a vector or mutant p53 expression plasmids. RT-qPCR was performed for Axl with cDNA from indicated cell lines. qPCR was performed for GAPDH and was used to normalize with Axl values. (B, D) Western blot analysis of Axl levels in H1299 cells stably transfected with a vector or mutant p53 expression plasmids. PAGE followed by Western blot analysis was performed with extracts made from cells stably transfected with a vector or mutant p53 expression plasmids. Immunoblots were developed for Axl (using an antibody against Axl from Abnova) and Erk2. Data show mutant p53–induced Axl expression both at RNA and protein levels in H1299 cells, and this induction is not disturbed by mutations at amino acids 22 and 23. Experiments were performed in technical triplicates. Experiments were performed multiple times with similar trends. Error bars showing standard deviations are indicated.