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. 2012 Oct 3;153(1):51–61. doi: 10.1093/jb/mvs118

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© The Authors 2012. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved

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Fig. 3 — Western blot analysis of the pro-LS-mat^WT and pro-LS-mat^S805A variants. Medium samples of COS-1 cells transfected with pSec-pro-LS-mat^WT alone (LS-WT), pSec-pro-LS-mat^WT and pSec-HAI-1^NIK1LK2 (LS-WT + HAI-1), pSec-pro-LS-mat^S805A alone (LS-S805A) and pSec-pro-LS-mat^S805A and pSec-HAI-1^NIK1LK2 (LS-S805A + HAI-1) were subjected to reducing SDS-PAGE. After western transfer, the blot was probed with Spr992 or S-protein–HRP. The 28-kDa position to which the SPCD part of two-chain pro-LS-mat^WT migrated is indicated as ‘SPCD of two-chain pro-LS-mat^WT’ to the right of the left panel. The 57-kDa position at which pro-LS-mat^S805A migrated is indicated as ‘pro-LS-mat^S805A’ to the right of the left panel. The 57-kDa position to which pro-LS-mat^WT and pro-LS-mat^S805A migrated is indicated as ‘Proforms’ to the right of the right panel. The position to which HAI-1^NIK1LK2 migrated is indicated to the right of the right panel. The molecular masses of the marker proteins are indicated in kilodaltons to the left of both panels.