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. 2012 Oct 3;153(1):51–61. doi: 10.1093/jb/mvs118

Fig. 4.

Fig. 4

Western blot analysis of the pro-S-matWT and pro-S-matS805A variants. Medium samples of COS-1 cells transfected with pSec-pro-S-matWT alone (S-WT), pSec-pro-S-matWT and pSec-HAI-1NIK1LK2 (S-WT + HAI-1), pSec-pro-S-matS805A alone (S-S805A) and pSec-pro-S-matS805A and pSec-HAI-1NIK1LK2 (S-S805A + HAI-1) were subjected to reducing SDS-PAGE. After western transfer, the blot was probed with Spr992 or S-protein–HRP. The 28-kDa position to which the SPCD part of two-chain pro-S-matWT migrated is indicated as ‘SPCD of two-chain pro-S-matWT’ to the right of the left panel. The 40-, 36- and 31-kDa positions to which pro-S-matWT and pro-S-matS805A species migrated are indicated as ‘Proforms’ to the right of the left and right panels. The position to which HAI-1NIK1LK2 migrated is indicated to the right of the right panel. The molecular masses of the marker proteins are indicated in kilodaltons to the left of both panels.