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. 2012 Dec 21;3:299. doi: 10.3389/fgene.2012.00299

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Copyright © 2012 Jiang, Zhou, Devarajan, Slater, Daly and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.

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Examples of validation of lincRNA CNV using qPCR. Methods of 2^−ΔΔCt and standard curve (SC) were compared. Assays from two levels of gDNA input were averaged and values were presented as the ratio of normalized tumor against normal sample using alpha-satellite and albumin as references. Stars represent copy number losses predicted by Nexus and detected by qPCR. Triangles represent copy number losses predicted by Nexus, but not detected by qPCR; a cutoff of 0.2 was used for qPCR (approximately 3 standard deviations of ALB normalized by alpha-satellite).