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. 2012 Sep 3;64(1):129–142. doi: 10.1093/jxb/ers236

Fig. 6.

Fig. 6.

Altered lignification in the fibrous roots from IbEXP1-antisense sweetpotato plants. (A) Histochemical analysis of lignin deposition in the fibrous roots from IbEXP1-antisense plants. Transverse hand-prepared sections of the fibrous roots from the wild-type (WT) and IbEXP1-antisense plants (#1 and #2) cultured in vitro for 3 weeks were stained with phloroglucinol-HCl. The stele areas of the FRs in the left panel are enlarged in the right panel. Lignified material is stained violet. EP, epidermis; CMX, central metaxylem; MX, metaxylem. (B) Transcript levels of the cinnamyl alcohol dehydrogenase (CAD) gene in IbEXP1-antisense plants. Total RNAs were extracted from fibrous roots of WT and IbEXP1-antisense plants (#1 and #2) cultured in vitro for 3 weeks. Real-time RT-PCR data were normalized to those for the endogenous β-tubulin gene. Error bars indicate the standard deviation (SD) between three technical replicates measured on fibrous roots collected from at least three different sweetpotato plants and subsequently pooled for analysis. (This figure is available in colour at JXB online.)