Fig. 5.

(A) In-gel detection of lipase activity in 3-h germinated pollen in culture medium with sucrose [(+)Su and without sucrose [(–)Su]. Major activity bands are indicated with arrows. The chart at the bottom display the average densitometric data corresponding to the lipolytic bands detected on three gels. (B) In-gel detection of lipoxygenase activity in 3-h germinated pollen in (+)Su and (–)Su culture media. Major activity bands are indicated with arrows. The chart at the bottom displays the average densitometric data corresponding to the lipoxygenase bands detected on three gels. Asterisks correspond to significant difference (P ≤ 0.01).