Fig. 5.

(A) Representative immunoblots of SDS-PAGE separated proteins from thylakoid membranes probed with antibodies raised against PSI (PsaA and PsaB) and PSII (D1, PsbO, Lhcb1)-associated polypeptides and Rubisco (PbcL) in control (C, 18 µM Mn) and Arabidopsis plants treated with 500 and 1500 µM Mn. (B) The relative polypeptide abundance was quantified by densitometric analysis (area × intensity bands) of the immunoblots and the presented data were normalized to the relative abundance of PsaA, PsaB, D1, PsbO, Lhcb1, and PbcL in control Arabidopsis plants. Mean ± SE were calculated from three independent experiments. Different lower-case letters indicate significant differences between the Mn treatments (P < 0.05).