Fig. 5.
NO production of 5-week-old wild-type Arabidopsis grown for 7 d in P-deficient nutrition solutions containing NO3 − or NH4 + exposed to ambient CO2 (350±50 µl l–1) or elevated CO2 (800±50 µl l–1). (a) Photographs of NO production shown as green fluorescence in the root tips and root-hair region of representative roots of plants supplied with NO3 − and exposed to ambient or elevated CO2, and with NH4 + exposed to ambient or elevated CO2. Bar, 500 µm. (b) Effects of N form and CO2 treatment on NO production expressed as relative fluorescence. NO production was visualized with DAF-FM DA dye. Data are means ±SD (n=15). Means followed by the same letter within a root segment are not significantly different at P <0.05.